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Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
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Genetic toggle switch to implement rewritable function
In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). We have to think two types of model - genetic circuit model and mathematical model - so as to construct PIGTS.
Genetic circuit model
fig5-1:genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure(PL is weakly induced)
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
fig5-2:Room pressure model
fig5-3:30MPa pressure model
We used two mathematical models and we call them 'room pressure model' and '30MPa pressure model'.
β30PL
We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function.
- Converge to high CI concentration state under 30MPa pressure (writable)
- Bistable under ordinary pressure (erasable)
fig5-4:Desirable condition
