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Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
From 2008.igem.org
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Genetic toggle switch to implement rewritable function
In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). We have to think two types of model - genetic circuit model and mathematical model - so as to construct PIGTS.
Genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure(PL is weakly induced)
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
We used two mathematical models and we call them 'room pressure model' and '30MPa pressure model'. The kinetics of the genetic toggle switch is calculated from 'room pressure model' under room pressure while that is calculated from '30MPa pressure model' under 30MPa pressure. In order to implement rewritable function, we use nullcline
- Converge to high CI concentration state under 30MPa pressure (writable)
- Bistable under ordinary pressure (erasable)
We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function.
Desirable condition
β30PL=1.
