Team:NTU-Singapore/Notebook/23 June 2008

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Monday 23 June

Morning (9am-1230pm)

  • DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on Sunday
  • Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
  • Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
  • Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.


Chin Chong

    • Preapre LBA agar plates of Top10 and BL21 containing LacI-GFP and allowed to incubate overnight at 37 deg cel
    • Designed the Lysis gene (with Promoter and RBS) and LsrA gene and requested for Quotations from companies
    • Inoculate top10 and BL21 cells containing LacI-GFP and grow in two seperate tubes containing 5 ml of LBA
      • allowed to incubate overnight at 37 deg cel
    • Autoclaved MgSO4, CaCl2 and M9 salts