Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white test

Piotr

1. The analysis of lacZ sequence from pZC320:
   There is no mutation in beta-galactosidase gene. Why are the colonies white?
2. Colony PCR.
   Template: DNA isolated from white colonies
   Primers: pZCseqL and pZCseqR