Team:Tokyo Tech/Construction

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Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Acknowledgements

Confirmatory experiment

figure1 We constructed A.PtetR-GFP, B.promoter less-GFP for confirmatory experiment

For confirming pressure-response ability of pressure-inducible promoter, we experimented under 0.1MPa and 30MPa pressure. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]) as a negative control.









figure2 Result of experiment

The result was that PtetR activity under 30MPa pressure is 2.5 times stronger than PtetR activity under 0.1MPa pressure.

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