Tests for ampicillin resistance given by protein added to medium

Piotr

1. Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).
2. Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (pACYC177+OmpA_A_omega twice as more as pACYC177+OmpA_omega with protein Z_alpha etc..)!!!!

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