Wiki/Team:Warsaw/vectors/pTrc99A-AID

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pTrc99A-AID vector

Description

This vector was a kind gift from:
Svend Petersen-Mahrt
DNA Editing Laboratory
Cancer Research UK
Clare Hall Laboratories
South Mimms Hert EN6 3LD
United Kingdom
44-1707-625770
44-20-7269 3801

Resistance: ampicillin
Features: Contains AID (Activation-Induced Deaminase) coding sequnece
Usage: All AID constructs were made basing on AID sequence taken from this vector. Was also used in tests for mutagenic activity of AID in E.coli cells

Vector map

Vector sequence

gtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtca
ggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactgca
taattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcg
ccgacatcataacggttctggcaaatattctgaaatgagctgttgacaat
taatcatccggctcgtataatgtgtggaattgtgagcggataacaatttc
acacaggaaacagaCCATGGACAGCCTCTTGATGAACCGGAGGAAGTTTC
TTTACCAATTCAAAAATGTCCGCTGGGCTAAGGGTCGGCGTGAGACCTAC
CTGTGCTACGTAGTGAAGAGGCGTGACAGTGCTACATCCTTTTCACTGGA
CTTTGGTTATCTTCGCAATAAGAACGGCTGCCACGTGGAATTGCTCTTCC
TCCGCTACATCTCGGACTGGGACCTAGACCCTGGCCGCTGCTACCGCGTC
ACCTGGTTCACCTCCTGGAGCCCCTGCTACGACTGTGCCCGACATGTGGC
CGACTTTCTGCGAGGGAACCCCAACCTCAGTCTGAGGATCTTCACCGCGC
GCCTCTACTTCTGTGAGGACCGCAAGGCTGAGCCCGAGGGGCTGCGGCGG
CTGCACCGCGCCGGGGTGCAAATAGCCATCATGACCTTCAAAGATTATTT
TTACTGCTGGAATACTTTTGTAGAAAACCATGAAAGAACTTTCAAAGCCT
GGGAAGGGCTGCATGAAAATTCAGTTCGTCTCTCCAGACAGCTTCGGCGC
ATCCTTTTGCCCCTGTATGAGGTTGATGACTTACGAGACGCATTTCGTAC
TTTGGGACTTTCTAGTTAATAAGAATTCGAGCTCCGTCGACAAGCTTggc
tgttttggcggatgagagaagattttcagcctgatacagattaaatcaga
acgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcgg
tggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgcc
gatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcgga
tttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgc
cataaactgccaggcatcaaattaagcagaaggccatcctgacggatggc
ctttttgcgtttctacaaactctttttgtttatttttctaaatacattca
aatatgtatccgctcatgagacaataaccctgataaatgcttcaataata
ttgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattc
ccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctg
gtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacat
cgaactggatctcaacagcggtaagatccttgagagttttcgccccgaag
aacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggta
ttatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacacta
ttctcagaatgacttggttgagtactcaccagtcacagaaaagcatctta
cggatggcatgacagtaagagaattatgcagtgctgccataaccatgagt
gataacactgcggccaacttacttctgacaacgatcggaggaccgaagga
gctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatc
gttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacacc
acgatgcctacagcaatggcaacaacgttgcgcaaactattaactggcga
actacttactctagcttcccggcaacaattaatagactggatggaggcgg
ataaagttgcaggaccacttctgcgctcggcccttccggctggctggttt
attgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgc
agcactggggccagatggtaagccctcccgtatcgtagttatctacacga
cggggagtcaggcaactatggatgaacgaaatagacagatcgctgagata
ggtgcctcactgattaagcattggtaactgtcagaccaagtttactcata
tatactttagattgatttaaaacttcatttttaatttaaaaggatctagg
tgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttt
tcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttg
agatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactcttttt
ccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttct
agtgtagccgtagttaggccaccacttcaagaactctgtagcaccgccta
catacctcgctctgctaatcctgttaccagtggctgctgccagtggcgat
aagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggc
gcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagc
gaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagc
gccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggt
atctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattt
ttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgc
ggcctttttacggttcctggccttttgctggccttttgctcacatgttct
ttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgag
tgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagt
gagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatc
tgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctct
gatgccgcatagttaagccagtatacactccgctatcgctacgtgactgg
gtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacg
ggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccg
ggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagg
cagcagatcaattcgcgcgcgaaggcgaagcggcatgcatttacgttgac
accatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccgga
agagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatg
tcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaac
caggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgat
ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggca
aacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcg
ccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgc
cagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaac
tatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaa
tgttccggcgttatttcttgatgtctctgaccagacacccatcaacagta
ttattttctcccatgaagacggtacgcgactgggcgtggagcatctggtc
gcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgt
ctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggt
tttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgat
gctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg
agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgat
accgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga
ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctc
agggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa
agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcagtgagcgcaacgcaattaatgtgagttagcgcgaattgatctg