Team:Chiba/Calendar-Home/18 September 2008

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17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
      • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • temparature
      37°C and 30°C

Team:Output

SAP 1μL

->left for:

  1. 1 hour at 37 degrees
  2. 30 minutes at 65 degrees.

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • Ligation product(Venus YFP)
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Sample No. each
DNA(μL) each
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
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