Team:Mississippi State/Check cell density
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Revision as of 20:51, 17 June 2008 by Caleb Dulaney (Talk | contribs)
To check cell density do the following:
- Zero the machine using same LB as used in the cell stock.
- Put 1ml Cell stock in vial for machine.
- Wipe off the outside of vial with tissue paper.
- Place vial in machine and close.
- Reading needs to be around 0.4, and at least above 0.3.
- If density doesn't meet this criteria, place back in 37C shaker for 5-10min. and check again.