Team:UNIPV-Pavia/Parts

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Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook


Assembled Parts Notation

In notebook section we sometimes use abbreviations to name assembled parts. New parts' official name, notebook abbreviations and new parts description are reported in the following table:


New Part Notebook Name Assembled Parts(code) Assembled Parts(name) Vector Description
- 01 J23100-E0240 Pcon-GFP_protein_generator J61002 Test
- 02 J23100-B0030 Pcon-RBS pSB1A2 Intermediate
- 03 R0051-B0030 Plambda-RBS pSB1A2 Intermediate
- 04 B0030-E0040 RBS-GFP pSB1A2 Intermediate
- 05 B0030-C0051 RBS-cI pSB1A2 Intermediate
- 06 23100-E1010 RBS-RFP pSB1A2 Intermediate
- 07 B0030-C0061 RBS-luxI pSB1A2 Intermediate
- 08 B0030-C0078 RBS-lasI pSB1A2 Intermediate
- 09 J23100-B0030-C0012 Pcon-RBS-lacI pSB1A2 Intermediate
- 10 J23100-B0030-C0040 Pcon-RBS-tetR pSB1A2 Intermediate
- 11 J23100-B0030-I15010 Pcon-RBS-cph8 pSB1A2 Intermediate
- 12 R0051-B0030-C0062 Plambda-RBS-luxR pSB1A2 Intermediate
- 13 B0030-E0040-B1006 RBS-GFP-T pSB1AK3 GFP protein generator with 39bp artificial terminator
- 14 B0030-C0051-B0030 RBS-cI-RBS pSB1A2 Intermediate
- 15 B0030-E1010-B1006 RBS-RFP-T pSB1AK3 GFP protein generator with 39bp artificial terminator
- 16 B0030-C0061-B1006 RBS-luxI-T pSB1AK3 luxI protein generator RCT
- 17 B0030-C0078-B1006 RBS-lasI-T pSB1AK3 lasI protein generator RCT
- 18 B0030-I15009 RBS-pcyA pSB1A2 Intermediate
- 19 J23100-B0030-C0012-B1006 Pcon-RBS-lacI-T pSB1AK3 lacI protein generator PRCT
- 20 J23100-B0030-C0040-B1006 Pcon-RBS-tetR-T pSB1AK3 tetR protein generator PCRT
- 21 J23100-B0030-I15010-B0030 Pcon-RBS-cph8-RBS ? Intermediate
- 22 R0051-B0030-C0062-B1006 Plambda-RBS-luxR-T pSB1AK3 Plambda regulated luxR protein generator PRCT
- 23 B0030-C0051-B0030-C0079 RBS-cI-RBS-lasR pSB1A2 Intermediate
- 24 B0030-C0061-B1006-R0062 RBS-luxI-T-Plux pSB1A2 Mux - Channel 0
- 25 B0030-C0078-B1006-R0079 RBS-lasI-T-Plas pSB1A2 Mux - Channel 1
- 26 B0030-I15009-B1006 RBS-pcyA-T pSB1AK3 pcyA protein generator RCT
- 27 J23100-B0030-C0012-B1006-R0010 Pcon-RBS-lacI-T-Plac pSB1AK3 lacI composite part: PRCT-Plac
- 28 J23100-B0030-C0040-B1006-R0040 Pcon-RBS-tetR-T-Ptet pSB1A2 tetR composite part: PRCT-Ptet
- 29 J23100-B0030-I15010-B0030-I15008 Pcon-RBS-cph8-RBS-ho1 ? Intermediate
- 30 R0051-B0030-C0062-B1006-R0062 Plambda-RBS-luxR-T-Plux pSB1A2 luxR composite part: Plambda regulated PRCT-Plux
- 31 B0030-C0051-B0030-C0079-B1006 RBS-cI-RBS-lasR-T pSB1AK3 cI-lasR protein generator RCRCT
- 32 B0030-I15009-B1006-R0082 RBS-pcyA-T-Pomp pSB1A2 pcyA composite part: RCT-Pomp
- 33 J23100-B0030-I15010-B0030-I15008-B0030-I15009-B1006-R0082 Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp ? Light responsive system under constitutive promoter: PRCRCRCT-Pomp
- 34 B0030-C0051-B0030-C0079-B1006-R0051-B0030-C0062-B1006-R0062 RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux pSB1AK3 Demux - Selector
- 35 B0030-C0051-B0030-C0079-B1006-R0051-B0030-C0062-B1006 RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T pSB1AK3 Mux - Selector
- 36 B0030-C0061-B0030-C0078-B1006-R0079 RBS-luxI-RBS-lasI-T-Plas pSB1A2 Demux - Input


  • 01 part has not been submitted to the registry because it was a test assembly.
  • Red-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
  • Green-coloured parts have been succesfully sequenced.
  • Blue-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct.
  • Red-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts have been succesfully tested and so we decided to submit them.
  • 24, 25, 34, 35, 36 are part of our final Mux or Demux systems.
  • Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages!
  • Parts in gray were programmed, but they have not been assembled and so they haven't been submitted.