Purification of proteins: A-alpha

Piotr, Emilia

1. Samples were resuspended in PBS, sonicated and centrifuged.
2. Lysis buffer was added separately to pellet and supernatant, then samples were boiled for 10 min.
3. Lysates loaded on 12% polyacrylamide gel (Fig. 1.) (amount relating to 100 μl of OD=1.0 culture).
4. Gel stained with Coomassie Blue. Optimal induction conditions chosen.
5. A-alpha inoculated for overnight culture (Rosetta strain).