Team:Warsaw/Calendar-Main/28 August 2008

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Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

  1. Colony PCR on colonies from plates with transformation pET15b+A_alpha.
    Primers used: AP+NotI and AL+SacI.
  2. Gel electrophoresis (Fig. 1).
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
Fig. 1. PCR on colonies from plates with transformation/b>
1. Marker
2-23. PCR products

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