Team:Heidelberg/Notebook/Sensing Group/Notebook/8thweek

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Contents

1 Monday, 09/22/2008
2 Tuesday, 09/23/2008
3 Wednesday, 09/24/2008
4 Thursday, 09/25/2008
5 Friday, 09/26/2008

Monday, 09/22/2008

test digestion of F1_LuxP_NdeI (F1 cloned in pDK48 with NdeI), F2_LuxP_NdeI, F1_LuxP_KpnI (F1 cloned in pDK48 with KpnI) and F2_LuxP_KpnI (F2 cloned in pDK48 with KpnI) with NcoI

Digestions of Fusion constructs with NcoI. Positive clones show two bands at 4308 and 2915 bp

Transformation of Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48) into MG1655 and HCB33 cells
Preparation of cultures for Maxipreps of Fusion constructs

Tuesday, 09/23/2008

Wednesday, 09/24/2008

Thursday, 09/25/2008

Friday, 09/26/2008

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