"
Team:Harvard/Dailybook/Week14/Chemical and Light
From 2008.igem.org
Mystery Colony PCR 10/08
Colony PCR of putative fungal colonies using primers BBpfx and mtrB500.
Lane 1: 1 kb ladder
Lanes 2-6: P136 plate (500 bp)
Lane 7: P140 plate (1.9 kb)
Lanes 8-12: P122>131 plate (1.3 kb)
Colony PCR 10/11
Unadulterated gel
Lanes 1-2: P148
Lane 3-4: P147 (picked 3)
Lanes 5-12: 147 (picked 6)
Lanes 13-14: P147
Lanes 15-24: P146 (picked 17, 23)
Lanes 25-26: P146
Lanes 27-36: P144 (picked 30, 35)
Lanes 37-39: P144
Lanes 40-48: P145 (picked 41, 46)
Ligations 10/10
| ' | Part 1 | Part 2 | Part 3 | Notes | ' | Total Volume | Growth |
| Ligation 1 | 123m4 ES | 131 XP | P1 EP | ts in Kan | just chem tran | ||
| 8 | 4 | 3 | 15 | ||||
| Ligation 2 | 122m4 Es | 131 XP | P1 EP | ts in kan | just chem tran | ||
| 8 | 4 | 3 | 15 | ||||
| Ligation 3 | 38+130 #20 ESX mmI | P1 ES | |||||
| 2ul to tube | 3 | ||||||
| Ligation 4 | 39+130 #31 ESX | P1 ES | |||||
| 10ul to insert tube | 35 | ||||||
| Ligation 5 | 142 Es | P1 ES | |||||
| 10ul to insert tube | 23 | ||||||
| Ligation 6 | 138 #2 ES | P1 ES | |||||
| 10ul to insert tube | 20 | ||||||
| Ligation 7 | 137 #32 ES | P1 ES | |||||
| 10ul to insert tube | 31 | ||||||
| Ligation 8 | 140 ES | P1 ES | |||||
| 10ul to insert tube | 20 | ||||||
| Ligation 9 | 131 XP | 116 SP | |||||
| 4 | 4 | 8 | |||||
| Ligation 10 | 131 XP | 117 SP | |||||
| 4 | 4 | 8 | |||||
| Ligation 11 | 131 XP | 108 Sp | |||||
| 4 | 4 | 8 |
