Made new culture of the PVJ4 transformation so that a miniprep can be done.
New culture of gene S105 part made.
Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.
Conductivity Tests
PVJ4 Colony 2 and 4 plus Controls. 10ml cultures. 420 ul of 50% Arabinose solution will be added. Conductance measurements will be taken before and after lysis has occurred.
Throughout the test, Optical Density measurements will be taken.