Tests for ampicillin resistance given by protein added to medium

Piotr

1. Purification of pACYC177+OmpA_alpha from antibiotic resistance contamination:
   Inoculation of E. coli TOP10 carrying pACTC177+OmpA_alpha to liquid LB with kanamycin and with kanamycin and ampicillin.
2. Inoculation of TOP10 carrying pACYC177+OmpA_A_alpha and pACYC177+OmpA-alpha to LB with inductor (0.25 mM IPTG) and kanamycin.

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Antoni

1. Transformation of E. coli TOP10 strain with friday's ligation products.
2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) were inoculated to liquid LB with kanamycin.