"
Team:Harvard/Dailybook/Week7/Widgetry
From 2008.igem.org
Revision as of 22:09, 26 October 2008 by Linchinlee (Talk | contribs)
Contents |
Goals for this week
- Shewanella and E. coli co-culture experiments
- If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
- Motivation:
- An inducible system in E. coli could be used to control Shewanella current production
Monday: August 4, 2008
Co-culture experiment setup
- Chambers 1 & 2: wt E. coli + wt Shewie + lactose
- Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose
Positive Controls
- Chamber 3: wt E. coli + wt Shewie + lactate
- Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate
Negative Controls
- Chamber 7: wt Shewie + lactose
- Chamber 8: wt E. coli + lactose
- Chamber 9: Lac-operon knockout E. coli + lactose
Tuesday: August 5, 2008
Co-culture experiment results, day 1
- Notes:
- At t = 2000 s, all cells added
- At t = 80000 s, all injection done
- Notes:
Zoomed-in graph of results, part 1
- Notes:
- Zoomed-in on time since injections
- Positive controls omitted
- Notes:
Preliminary Conclusions
- Noticeable difference in behavior between wt E. coli + Shewie and Lac-operon knockout + Shewie co-cultures
- Positive and negative controls show most likely no other variables involved
- Could be used to create tic-tac-toe which uses the difference in behavior to determine moves
