"

 

From 2008.igem.org

12^th week

go back to 11^th week

go back to the overview

Contents 1 Monday 10/20/2008 1.1 pSB1A3-Receiver-Colicin cloning 1.2 Sender cloning: constitutive promotor-sender 2 Tuesday 10/21/2008 2.1 Sequencing results of ready cloned BioBrick parts 2.2 pSB1A2-Receiver-Colicin cloning 2.3 HisTag cloning of Colicins for purification 2.4 Sender cloning: pBAD-sender 2.5 Sender cloning: constitutive promotor-sender 3 Wednesday 10/22/2008 3.1 pSB1A3-Receiver-Colicin cloning 3.2 HisTag cloning of Colicins for purification 4 Thursday 10/23/2008 4.1 pSB1A2-Receiver-Colicin cloning 4.2 HisTag cloning of Colicins for purification 4.3 Colicin activity test 5 Friday 10/24/2008 5.1 pSB1A3-Receiver-Colicin cloning 5.2 HisTag cloning of Colicins for purification 5.3 Sender Cloning: pBAD - sender 5.4 Sender Cloning: constitutive promotor - sender 6 Saturday 10/25/2008 6.1 pSB1A3-Receiver-Colicin cloning 6.2 HisTag cloning of Colicins for purification 6.3 Sender Cloning: pBAD - sender 6.4 Sender Cloning: constitutive promotor - sender 7 Sunday 10/26/2008 7.1 pSB1A3-Receiver-Colicin cloning 7.2 Sender Cloning: pBAD - sender 7.3 Sender Cloning: constitutive promotor - sender

Monday 10/20/2008

pSB1A3-Receiver-Colicin cloning

• Minipreps of colE1/E9/E9lys-Receiver with QiaCube, Qiagen
• Send probes to GATC for sequencing

Sender cloning: constitutive promotor-sender

• Minipreps of J23107-Receiver with QiaCube, Qiagen
• Send probe to GATC for sequencing

Tuesday 10/21/2008

Sequencing results of ready cloned BioBrick parts

	EcoRI-site mutation	PstI-site 1 mutation	PstI-site 2 mutation	PstI-site 3 mutation	Prefix	Suffix	complete sequence
colE1_BB_57-1	+	+	+	+	+	+	+
colE1_BB_57-2	+	+	+	+	+	+	+
colE1_BB_57+2	?	+	+	?	+	?	missing sequence
colE1_BB_67+1	+	+	+	+	+	?	sequencing failure
colE9_BB_(2)	+	XXX	XXX	XXX	+	+	+
colE9lys_BB	XXX	XXX	XXX	XXX	+	+	+
sender_BB	XXX	XXX	XXX	XXX	+	+	+

--> colE1_BB_57-1, colE9_BB_(2), colE9lys_BB and sender_BB gave positive sequencing results in all criteria and will be sent to the registry

pSB1A2-Receiver-Colicin cloning

HisTag cloning of Colicins for purification

Sender cloning: pBAD-sender

Sender cloning: constitutive promotor-sender

Wednesday 10/22/2008

pSB1A3-Receiver-Colicin cloning

HisTag cloning of Colicins for purification

Thursday 10/23/2008

pSB1A2-Receiver-Colicin cloning

HisTag cloning of Colicins for purification

Colicin activity test

Friday 10/24/2008

pSB1A3-Receiver-Colicin cloning

HisTag cloning of Colicins for purification

Sender Cloning: pBAD - sender

Sender Cloning: constitutive promotor - sender

Saturday 10/25/2008

pSB1A3-Receiver-Colicin cloning

HisTag cloning of Colicins for purification

Sender Cloning: pBAD - sender

Sender Cloning: constitutive promotor - sender

Sunday 10/26/2008

pSB1A3-Receiver-Colicin cloning

Sender Cloning: pBAD - sender

Sender Cloning: constitutive promotor - sender

go to 13^th week

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Permanent link
• Privacy policy
• Disclaimers