From 2008.igem.org
pBca1256-K112300
PCR al0001/al0002 on pSB3C6-Bjh1501 (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
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al0001 Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002 Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
pBca1256-K112301
PCR al0003/al0002 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
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al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
pBca1256-K112302
PCR al0001/al0004 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
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al0001 Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004 Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc
pBca1256-K112303
PCR al0003/al0004 on pSB3C6-Bjh1501 (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
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al0003 Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc
pBca1256-K112304
PCR al0005/al0002 on pSB3C6-Bjh1501 (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
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al0005 Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002 Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
pBca1256-K112305
PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
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al0006 Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002 Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
pBca1256-K112306
PCR al0007/al0008 on pSB3C6-Bjh1502 (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
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al0007 Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0008 Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112307
PCR al0009/al0008 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
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al0009 Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008 Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112308
PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
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al0007 Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010 Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc
pBca1256-K112309
PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009 Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010 Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc
pBca1256-K112310
PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
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al0011 Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008 Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112311
PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
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al0012 Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008 Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112312
PCR al0013/al0008 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
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al0013 Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0008 Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112313
PCR al0014/al0008 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
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al0014 Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0008 Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112314
PCR al0013/al0010 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
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al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0010 Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc
pBca1256-K112315
PCR al0014/al0010 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
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al0014 Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0010 Reverse Biobricking of lambda antiholin with no start and stop codons
CGttaGGATCCttgatttctaccatcttctactccggc
pBca1256-K112316
PCR al0015/al0008 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
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al0015 Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
al0008 Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112317
PCR al0016/al0008 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
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al0016 Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
al0008 Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc
pBca1256-K112318
PCR al0017/al0018 on MG1655 (467bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
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al0017 Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
al0018 Reverse Biobricking of bolA promoter
CGttaGGATCCcttctatccgctcacgtatc
pBca1256-K112319
PCR al0019/al0020 on MG1655 (465bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112319
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al0019 Forward Biobricking of ftsQ promoter
CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg
al0020 Reverse Biobricking of ftsQ promoter
CGttaGGATCCcctcttcttcgctgtttcgc
al0023 Forward Removing of EcoRI
gtagtacgaattgtggaactggcg
al0024 Reverse Removing of EcoRI
cgccagttccacaattcgtactac
pBca1256-K112320
PCR al0021/al0022 on MG1655 (804bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
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al0021 Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
al0022 Reverse Biobricking of ftsAZ promoter
CGttaGGATCCgtaccaatctccagtcctactaccag
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