Preparation of AraC+pBAD+AID

Michał K.

1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AraC+pBAD+AID.
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of BioBricks

Michał K.

1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_alpha_ and RFP(?????) + AraC+pBAD+AID.
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found only for pACYC177 + OmpA_alpha_.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.