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From 2008.igem.org

Contents 1 Results: Current Status and Tests 1.1 Cellulolysis device 1.2 Glycogenesis device 1.3 Starch synthesis device 1.4 Lycopene generator 1.5 Limonene synthase device

Results: Current Status and Tests

Cellulolysis device

• cenA (endoglucanase) and cex (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
• bglX (beta glucosidase): Biobrick made.
• P_cstA (promoter): Biobrick made and tested - successful.
  • P_cstA promoter assay with xylE as repoter

Glycogenesis device

• glgC and glgC16 (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful.
  • Glycogen Assay 1 (Qualitative)
  • Glycogen Assay 2 (Qualitative)
  • Glycogen Assay 3 (Quantitative)

Starch synthesis device

• su1 and iso2 (isoamylases): Work in progress - to be continued in an undergraduate honours project.

Lycopene generator

• dxs+crtE+crtB+crtI+crtY: Work in progress - encountered problem at final step.

Limonene synthase device

• dxs+LIMS+appY: Biobrick made - still being tested (last test failed to detect limonene synthesis).

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