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Team:Edinbrugh/Results/Glycogen 4
From 2008.igem.org
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Glycogen Assay 4 (Quantitative: Raman)
Raman spectroscopy was performed by Dr Rabah Mouras.
Experiment Design
See Glycogen Assay 3 for explanation.
Legend
Organisms:
- Organism 1: Control strain of E. coli containing a plasmid that should have no effect on glycogen levels.
- Organism 2: E. coli overexpressing glgC.
- Organism 3: E. coli overexpressing glgC16.
Growth Conditions:
- Condition A: normal growth medium (LB, or L-broth).
- Condition B: LB + 10 mM glucose.
- Condition C: LB + 25 mM glucose.
- Condition D: LB + 50 mM glucose.
It is expected that the presence of higher levels of glucose, up to a point, will increase the amount of glycogen produced by the cells.
Results
Analysis forthcoming.
