Team:NTU-Singapore/Notebook/29 May 2008

ChinChong (~~)

• • Requested E coli strain B2B110 with the ColE7 plasmid from Professor David Gordon (Australia)
  • Requested quotation from Invitrogen on some chemicals and kits
  • Discussed possible experiments design to patch up the parts of our BioBrick
  • Finding parameters for the modeling from literature

Darius

• • Come up with provisional LacI experiment design
  • Discussed possible experiments design to patch up the parts of our BioBrick

Choon Kit

• • Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI
  • Alias with Professor Yuan Hanna (Taiwan)to get E7+Imm plasmid, expected arrival 5th Jun 08
  • Alias with Professor Chris Viogt (UCSF) to get AND modular plasmids, expected arrival 5th Jun 08
  • Requested full DNA sequence of ColE7 and Imm plasmid from Zhong Zhi Hao (Taiwan)
  • Requested full DNA sequence of AND modular plasmids from Chris Voigt (UCSF)
  • Documented laboratory work for 28 and 29 May 2008

Hung

• • Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI (basically phase II and up to step 3 of phase III of experiment 3 for iron promoter. We left the DNAs incubated overnight instead of for 2hrs due to time constraint.)
  • Finding parameters for the modeling from literature

ZhenFu

• • Improve our group's model with some literature parameters
  • Added equations to our model
  • Puzzled over results of simulation, shall work on it further with other members of the group