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Team:University of Lethbridge/Notebook/Project3October
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October 21 2008
Andrew
Transformation of +18 rpsA TIR DNA into pGEM TEZ to make enough DNA to be restricted/ligated into biobrick format. (psB1A2)
50 uL og xgal added to LB + amp plates to screen for blue and white colonies.
October 22 2008
Andrew
Transformation into pGEM TEZ successful, many white and some blue colonie on both plates.
Picked 3 colonies from each plate to grow in 5mL LB + amp for a pDNA prep.
October 23 2008
Andrew
pDNA prep of 6 culture tubes using a kit
