Team:Warsaw/Calendar-Main/30 September 2008
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Mutagenesis of protein APawełResults of sequencing: unfortunately all sequences were wild-type. Preparation of vectors for BiobricksMichał K., Piotr
1. Marker 2-3. digested plasmids BBa_I739204 4. digested plasmid psB2K3 Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of OmpA-linker (BBa_K103006)Piotr
Preparation of omega_linker under PT7 (BBa_K103020)Michał K.
Preparation of Z(BBa_K103004)Piotr
Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)Michał K.Overnight digest of purified BBa_K103018 fragment (from previous day) with EcoRI (EcoRI buffer). DNA ends blunting with Klenow fragment. Fig. 2. BamHI/PstI digests of isolated plasmid1. Marker 2. digested plasmid pACYC_OmpA_omega_deltaA_alpha
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