Team:NTU-Singapore/Notebook/25 June 2008
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Wednesday 25 June
Zhen Fu
take out the 50ml tubes incuated from Tuesday evening. Prepare the varying lactose concentratons (1 to 10 mM) as well as the controls in 1.5ml eppendorh tubes as preperations for Characterization Chin Chong
Diluted the cells(Top 10 and BL21) from the 50ml tubes to OD 0.590 and re-incubate them to allow them to grow to 1.2
Hung
take out the agar plates incubated from Tuesday night. For details of the plasmids, please refer to Tuesday notebook
Choon Kit
digestion for:
Chin Chong, Zhen Fu
Proceed with the characterization experiment
Hung, Min-Lin
Transform these following plasmids to Homemade top10 cells for amplification and obtaining new plasmids with higher purity (using new Miniprep Kit):
Chin Chong, Zhen Fu
Hung
Choon Kit3pm to 7pm:
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