Team:Chiba/AHL Receiver Phase

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Chiba-U.gif

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Signal Molecule Receiver Phase

Quorum_Sensing_Crosstalk

Design

Table.1 LuxR family gene

センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）


Experiment

Fig.R family crosstalk test
  • Sender
    • AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]
LuxI-sender Chiba.gif


  • Receivers
    • LuxR

Non-AiiA-Receiver Chiba.gif

    • RhlR[http://partsregistry.org/Part:BBa_K084004 (BBa_K084004)]

RhlR-p15A Chiba.gif

    • LasR[http://partsregistry.org/Part:BBa_K084005 (BBa_K084005)]

LasR-p15A Chiba.gif

    • CinR[http://partsregistry.org/Part:BBa_K084006 (BBa_K084006)]

CinR-p15A Chiba.gif

  • Reporter
    • Plux-GFP[http://partsregistry.org/Part:BBa_J37032 (BBa_J37032)]

Plux-GFP-pMB1 Chiba.gif



Result

R-family-crosstalk-result Chiba.gif



Plasmid_Copy_number

Design

Experiment

Result

Copy-nomber-change-result-detail version Chiba.gif

LuxR_mutant

Design

Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)


Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))

私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)


Experiment

Result

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