Team:Heidelberg/Notebook/material
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SM | 50 mM | Tris, pH 7.5 |
20 mM | MgSO4 | |
50 mM | NaCl | |
0.01 % | gelatine | |
Tethering buffer, pH 7.0 | 10 mM | potassium phosphate |
(K2HPO4 : KH2PO4 = 1 :1 ) | ||
100 µM | EDTA | |
1 µM | L-Methionine | |
10 mM | lactic acid | |
M63 salt | 3 g/l | KH2PO4 |
9 g/l | K2HPO4 | |
4 g/l | (NH4)2SO4 | |
0.5 g/l | FeSO4 | |
Amino acid mix | 5 g/l | L-threonine |
5 g/l | L-histidin | |
5 g/l | L-leucine | |
5 g/l | L-methionine | |
M9 salts | 64 g/l | Na2HPO4 x 7H2O |
15 g/l | KH2PO4 | |
2.5 g/l | NaCl | |
5 g/l | NH4Cl |