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Team:NTU-Singapore/Notebook/23 June 2008
From 2008.igem.org
Monday 23 June
Morning (9am-1230pm)
- DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on Sunday
- Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
- Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
- Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.
