Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

1. There were blue and white colonies but no red or green in UV.
2. Inoculation of liquid LB medium + Amp with white colonies.
3. Inoculation of 10ml LB with E.coli TOP10 carrying pZC320.
4. Inoculation of E.coli TOP10 carrying pSB1A2 standard plasmids carrying parts: BBa_E0840(GFP genetrator) and BBa_J04450 (RFP generator).

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Inoculation of E. coli with pET15b plasmid on LB + ampicillin.