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Team:Edinburgh/Results/BABEL
From 2008.igem.org
BABEL: BioBrick Assembly with blunt-ended ligation
BABEL is an alternative, restriction enzyme-free method for generating BioBricks. It is fully compatible with the BioBrick 1.0 standard and also with all of the proposed successor standards, and is itself a potential successor to the BioBrick 1.0 standard in that it can be used for fully scarless assembly. Babel is based on blunt-ended ligation of PCR products followed by fusion PCR and self-ligation. Here we describe the use of BABEL to generate fully compliant BioBricks in pSB1A2, a procedure referred to in our laboratory as 'SOB', or 'Son Of Babel'. Possible extensions of this concept to scarless assembly should be obvious. The 'Son of Babel' procedure is as follows:
- Generate a blunt-ended linear version of pSB1A2 by PCR using appropriate primers which contain the entire BioBrick prefix and suffix.
