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Team:Chiba/AHL Receiver Phase
From 2008.igem.org
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Signal Molecule Receiver Phase
Quorum Sensing Crosstalk
Design
Table.1 LuxR family gene
センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）
Experiment
- Sender
- AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]
Fig.R family crosstalk test
- Receivers
- LuxR
- RhlR[http://partsregistry.org/Part:BBa_K084004 (BBa_K084004)]
- LasR[http://partsregistry.org/Part:BBa_K084005 (BBa_K084005)]
- CinR[http://partsregistry.org/Part:BBa_K084006 (BBa_K084006)]
- Reporter
- Plux-GFP[http://partsregistry.org/Part:BBa_J37032 (BBa_J37032)]
Result
Fig. Time Delay Test
Plasmid Copy number
Design
Experiment
Sender
- AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
- Low Copy Receiver
Result
Fig. Time Delay Test
LuxR_mutant
Design
Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)
Experiment
Result
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