Team:Hawaii/Protocols/Transformation E coli

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Protocol

  1. Thawed cells on ice.
  2. Transformed 50 μl cells w/ 1 μl plasmid
  3. Incubated on ice 30 min.
  4. Heat shocked 60 sec. in 42C water bath
  5. Added 250 μl SOC
  6. Incubated @ 37C for 1 hour with shaking (150 rpm)
  7. Plated 20 μl onto LB+spectinomycin plates
  • Plate more if needed.
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