Team:Cambridge/Signalling/Lab Work

From 2008.igem.org

Revision as of 17:22, 28 October 2008 by 06chapar (Talk | contribs)

Agr A and C ligation to pSB4C5

Ligation was performed using Fermentas Rapid Ligation kit according to their protocol.

After ligation, samples were visualized on a gel, but little product of the correct size was seen.

For agrA we should have gotten a band of size 3700 (3000plasmid + 700 insert)

For agrC we should have gotten a band of size 4300 (3000plasmid + 1300insert)


The bands of appropriate sizes were extracted for transformation.





Retrieved from "http://2008.igem.org/Team:Cambridge/Signalling/Lab_Work"