Sequence

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Sequencing Protocol

Protocol:

Go to Genewiz.com to order sequencing.

Sequencing primers should be diluted to 5uM; 4.0ul of 5uM primer should be used.

Depending on plasmid size and concentration (see table in Protocol.pdf), use up to 8ul of DNA; if less than 8ul, add autoclaved deionized water.

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