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Team:Harvard/Hardware
From 2008.igem.org
Motivation
The broad goal of our project was to engineer s. Odenisis to produce a detectable change in electric current in response to some environmental stimulus. In order to observe such a reaction, our first task was to design an environment capable of housing bacteria and measuring current production.
Solution - Microbial Fuel Cells
Background
Microbial Fuel Cells (MFCs) are devices that use bacteria as the catalysts to oxidize organic and inorganic matter and generate current <ref></ref>. They have been used by researchers to study the mechanisms involved... The principle behind these devices is to physically separate the oxidation and reduction reactions, creating an electrical path between
Context
Design Goal
Functional description
The final product is a complete system capable of introducing separate strains of bacteria to multiple different environments while simultaneously measuring and recording current readings from each. The experimenter specifies the number of bacteria/environment combinations to be run, as well as the initial conditions for each. Data collection and storage is automated, with a computer displaying live current readings and graphing historical current levels. The experimenter can change the conditions of any fuel cell throughout the course of the experiment without affecting other fuel cells. The fuel cells themselves are stand-alone, capable of being treated as individual circuit components.
Specifications
- automated -- experiments can last several days. measurements must be automated to allow for overnight observation.
- anaerobic/aerobic -- s. Odenisis only oxidizes substrates in anaerobic environments. The chamber housing the bacteria must be oxygen free and airtight.
- sterile -- fuel cells must be capable of being sterilized to prevent contamination
- reproducible -- individual fuel cells must be similar enough to produce consistent results.
- accessible -- experimenters must have access to the bacterial environment.
Approach
Decomposition into components
Construction of the system can be broken down into three distinct parts. The most important components are the fuel cells themselves. Once these devices have been built, a measurement system must be constructed to sample current readings
Component descriptions and approaches
