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From 2008.igem.org

Contents 1 Running an MFC Experiment from Start to Finish 1.1 Creating a Testing Environment 1.1.1 Constructing Fuel Cell Components 1.1.2 Setup of Digital Multimeter 1.1.3 Controlling the DMM with LabView™ 1.2 Experiment Preparation 1.2.1 Assembling Chambers 1.2.2 Solutions Prep 1.2.3 Gas Tubing Assembly 1.2.4 Growing Strains 1.3 Runtime 1.3.1 Bacteria 1.3.2 Fuel Cells 1.3.3 Injections/Variables 1.4 Clean Up

Running an MFC Experiment from Start to Finish

This page is intended as a comprehensive guide to completing a microbial fuel cell experiment

Creating a Testing Environment

Begin 1-2 weeks prior to experiment

Constructing Fuel Cell Components

Setup of Digital Multimeter

Controlling the DMM with LabView™

Experiment Preparation

Begin 1 day prior to experiment

Assembling Chambers

Solutions Prep

Chamber media

• 5.844 g/L 100mM NaCl
• 15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

• 2.918 g/L Monosodium phosphate, monohydrate
• 4.095 g/L Disodium phosphate, anhydrous
• 5.844 g/L 100mM NaCl

7.0 pH

Gas Tubing Assembly

Growing Strains

Runtime

Begin 2 hours prior to experiment

Bacteria

Fuel Cells

Injections/Variables

Clean Up

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