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Team:University of Ottawa/12 June 2008
From 2008.igem.org
Contents |
Today in the lab
Dan and Matt
- Results from the gel
- Showed that PCR did not work
- Source of problems can be from:
- 1. The sequence that we have on hand is not the plasmid that we have
- To resolve this we will digest the plasmid with HindIII
- 2. The PCR is not working
- We will run a PCR temperature gradient.
- Source of problems can be from:
- If the second test is inconclusive we will order new primers.
Digestion of 7/8 plasmids using HindIII'
- Worked out as expected, there is a high probability that we have the correct plasmid
PCR gradient between 55 and 70 degrees C
- Gel showed that there are very faint expected PCR products between ~56-60 degrees C
- We will narrow our gradient and try the PCR optimization again, although we think that it is unlikely that there is a problem with the PCR because we are using the enhanced phusion enzyme.
