Team:The University of Alberta/Parts

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BioBrick.jpg

Contents

Butanol Parts

Parts Submitted

  • [http://partsregistry.org/Part:BBa_S04142 21 + 22] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)

Original Parts Used This Summer

  • [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. 21)
  • [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. 22)
  • [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. 23)
  • [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. 24)
  • [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. 25)
  • [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
  • [http://partsregistry.org/Part:BBa_I0500 I0500]
  • [http://partsregistry.org/Part:pSB1A3 pSB1A3]

Parts Ordered This Summer

  • ETFA (electron-transfer-flavoprotein, alpha polypeptide)
  • ETFB (electron-transfer-flavoprotein, beta polypeptide)

Parts Assembled This Summer

For Protein Expression
  • J61003 + 21
  • J61003 + 22
  • J61003 + 23
  • J61003 + 24
  • J61003 + 25
  • I0500 + 21
  • I0500 + 22
  • I0500 + 23
  • I0500 + 24
  • I0500 + 25
For Butanol Operon
  • 21 + 22 (in plasmid pSB1A3)
  • 21 + 22 + 23 (in plasmid pSB1A3)
  • 23 + 24 (in plasmid pSB1A3)
  • 24 + 25 (in plasmid pSB1A3)
  • 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
  • ETFA + ETFB (in plasmid Puc57)


Plant Parts

Plant Banner.jpg

  • Plant CAMV 35S Promoter
  • Plant Resistance Brick
    • Chloramphenicol was used
  • IGEM Binary Vector
    • We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
  • Blue OX, Purple Russian reporter systems

Plastic Project

Plastic Banner.jpg

  • TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
  • PLACIQ + ERE brick - Sequence
  • Estrogen Receptor Alpha (Estrogen binding domain)
  • Estrogen Receptor Alpha (DNA binding domain)
  • BisdA BPA degredation bio brick - Sequence
  • BisdB BPA degredation bio brick - Sequence

Parts Ordered

PDF Sequences of synthetic genes ordered 20 Jun

Genbank files synthetic genes ordered 20 Jun
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