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Team:University of Ottawa/2 July 2008
From 2008.igem.org
Contents |
Today in the Lab
Matt
- Ligation
- I am using three different samples of 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42.
- A gel confirmation was run however nothing appeared on the gel due to a very low DNA concentration of the PTP2.
- We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning.
- Transformation
- The competent cells were transformed with the ligation product of all three samples and left overnight.The receptor component Atcre was also integrated into competent cells.
- Dehydrogenase component
- The dehydrogenase was received in the mail, I streaked the Ecoli containing the component to grow overnight in the incubator. Tomorrow we will inoculate for mini prep the following day.
Dan
- PCR of pSSRE constucts
- Turned out to be a disaster. Had nonspecific binding and >4 bands. We would be better of going back to the original gel extraction products.
- Digestion
- Now I am using the original gel extraction products (1,2,3,4) and
