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From 2008.igem.org

Contents 1 LB agar 1.1 Materials 1.2 Procedure 2 LB + amp100 2.1 Materials 3 LB+kan20+amp50 3.1 Materials

LB agar

Materials

• 25 g LB broth mixture (Difco)
• 1 L ddH₂O

Procedure

1. Mix dry ingredients together with 1 L ddH₂O using a magnetic stir bar.
2. Autoclave to sterilize. Let cool to 55C.
3. Pour 28 ml LB solution for thin plates; 50 ml for thick plates.

LB + amp₁₀₀

Materials

1 L LB agar 1 ml 1000X ampicillin stock solution

1. Make LB agar as directed.
2. When mixture is cooled to 55C, add 1 ml 1000X ampicilllin stock solution.
3. Plate as usual.

LB+kan₂₀+amp₅₀

Materials

1 L LB agar 0.5 mL 1000X ampicillin stock solution

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