Team:NTU-Singapore/Notebook/25 June 2008

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Wednesday 25 June

1030am: Hung: take out the agar plates incubated from Tuesday night. For details of the plasmids, please refer to Tuesday notebook
- GFP reporters with standard, weak, medium and strong promoters all have colonies.
- GFP reporter device BBa_E2040 however shows no colony.
- GFP producer BBa_E4080 shows a few colonies.
- LacI-GFP shows no colony.
- GFP self-ligation shows a few colonies.
- pFe-GFP shows no colony.

11am: Group meeting to discuss about current obstacles encountered during wetlab.

12:15pm: Choon Kit: digestion for:
- pFe with SpeI/PstI
- GFP with XbaI/PstI
- pFe with SpeI to check SpeI activity
- Terminator with SpeI to check SpeI activity
  Incubate for 3 hours.

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