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File:Fig 4-cloningstrategy colicinreceivers.png
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Cloning strategy of the LuxR-colicin-receiver parts (E1: BBa_K150009, E9 long: BBa_K150011, E9 short: BBa_K150013) First of all the LuxR receiver part (BBa_T9002) was amplified by PCR. The product, containing a Ptet promoter, the luxR gene followed by the PluxR promoter and a ribosome binding site was cloned into pSB1A2 vector. This vector was extracted from BBa_T9002. In a second cloning step the different colicin operons, amplified from pColE1 or pColE9-J were cloned behind the PLuxR promoter.
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| current | 01:30, 29 October 2008 | | 3,867×3,029 (947 KB) | Andreaskuehne (Talk | contribs) | (Cloning strategy of the LuxR-colicin-receiver parts (E1: BBa_K150009, E9 long: BBa_K150011, E9 short: BBa_K150013) First of all the LuxR receiver part (BBa_T9002) was amplified by PCR. The product, containing a Ptet promoter, the luxR gene followed by the) |
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