Team:Warsaw/Calendar-Main/23 July 2008
From 2008.igem.org
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Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPawełLigations transformed into TOP10 and plated on LB + ampicillin. Preparation of chemocompetent E. coli TOP10AntoniSetup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria. Cloning of protein A DNA to OmpA constructsMichał K.
![]() 1-4. digested plasmids isolated from transformants obtained previous day 5. Marker
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