Harvested the DNA from the overnight colonies.
Used this DNA in a PCR sequencing reaction.
The product was wrapped in foil (to keep out the light) and stored at 4C overnight. Team Fungus:
Ran PCR3 product out on 1% agarose gel. Ran side by side gels to test gel boxes.
Sequencing primers arrived.
Purified PCR product from PCR2 from 7/7.
Purified double digested insert from 7/3.
Made LB+Amp plates.
Received lignin peroxidase from Sigma.
Purified pET28a from overnight cultures.
Purified PCR product form 7/7 PCR3.
Ran PCR3 out on 1% agarose gel.
Isolated genomic DNA from BL21.
Purified PCR product form 7/7 PCR2.
Ligated purified PCR2 product into PGEM-T easy.
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