Wisconsin: Lignin Project/11 July 2008

Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use.
Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit.
created chemically competent stocks of MG1655, JW3890 and RL357.
The stocks were frozen down and kept at -80C for future use.
changing growth temperature increased growth of tpiA knockout greatly.
Team Fungus
Researched the necessary environment for the disulfide bond formation needed in correct folding of LiP
Concluded that a oxidative cytoplasm was needed, thus the Novagen Origami strains were chosen as final transformation hosts, specifically Rosetta-gami2(DE3).
Ran out gels of all tubes in freezer. Found five tubes with correct size bands.