Team:The University of Alberta/30 September 2008

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  • Tom

can't tell much from the gel2
gel1. expected size 3000bp (2-9) and 2000bp(10-16). this is as expected
gel2. expected size 4000bp (1-8) and 4200bp(10-16). result is as expected, but have some short band(contaminations?)
they all seems good! have to sequence to make sure.


David

starting the bisdA and bisdB cassettes from scratch because i really, really, really want to see if this stuff will work.

i digested 

 the correct pTET vector complete with RBS site and 6xHis tag (pTET) with SphI+PstI+CIP treatment
 BisdA with XbaI+PstI
 BisdB with XbaI+PstI

i gel purified the products and set up the following ligations 

 pTET+BisdA
 pTET+BisdB
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