Team:The University of Alberta/2 October 2008

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Tom
- ETFA/B already have RBS site, they are ready for use
- setup overnight of 21-25(1-4); ETFA/B (5-6)
- redo 21-25 ligation tomorrow
- religate 21+22+23(S/P) AND 24+25(X/P)
- sequence ETFA/B with Vf and Vr

David

i picked candidate colonies from yesterdays transformations to grow up overnight in LB amp.

 pTET+BisdA
 pTET+BisdB

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