Team:The University of Alberta/9 October 2008
From 2008.igem.org
- Tom
- run the colony PCR on gel
- get size: 21-25 5.5Kb, ETFA 2kb and 2.8kb
- digest PCR with X/P
- run the colony PCR on gel
David
i miniprepped yesterdays cultures and checked them with NotI. they worked. we now have pUC57-pTET-BisdA+pTET-BisdB.