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Team:UCSF/Parts
From 2008.igem.org
UCSF iGEM 2008 Parts
For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1
The bulk of our parts/constructs were generated using the AarI cloning technique: Everything_you_ever_wanted_to_know_about_AarI
AarI Shuttle Vector
So you don't want to use AarI cloning? OK. We've provided a shuttle vector that allows one-sublcone conversion of AarI parts into biobricks.
- AarI--Biobrick Shuttle AB
- AarI--Biobrick Shuttle BD
more details: Everything_you_ever_wanted_to_know_about_AarI
Project-relevant AarI Parts
digestion of these plasmids with AarI will release a fragment for AarI cloning.
- LexA (1-87 a.a) AB
- Sir2 BD
- GFP AD
- Ssn8 AD
- Sir3 A!D
- Sir4 AD
AarI acceptor vectors (empty) These pRS3series vectors accept AarI digested parts between a promoter/terminator. The promoter is flanked by PspOMI/XhoI sites, and the terminator by Not1/SacI sites, in case you want to change them out.
- AarI AD Acceptor (pRS305, Gal1P, Adh1t)
- AarI AD Acceptor (pRS315, Adh1P, Adh1t)
- AarI AD Acceptor (pRS315, Cyc1P, Adh1t)
- AarI AD Acceptor (pRS315, Fig1P, Adh1t)
- AarI AD Acceptor (pRS315, 8X LexA Ops-Cyc1P, Adh1t)
- AarI AD acceptor (pRS315, Cyc1P, Adh1t-8XLexA Ops)
- AarI AD Acceptor (pRS315, 8X LexA Ops-Fig1P, Adh1t)
Bonus AarI Parts
These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.
Sir2 AB
Esa1 AB (Histone Acetyltransferase)
Sas2 AB (Histone Acetylatransferase)
Sas2 BD
mCherry AB
mCherry BD
GFP AB
GFP BD
LexA (1-87) BD
TetR AB (Tet Repressor DNA Binding Domain)
TetR BD
LacI AB (Lac Repressor DNA Binding Domain)
Pif3 AB (Light-inducible dimerization partner)
PhyB AB (Light-inducible dimerization partner)
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